Sign in →

Test Code 5422463 IDH1 AND IDH2 MUTATIONS FFPE

Test ID

1236000025

Specimen Requirements

Collection Requirements Ten unstained 10-micron thick paraffinized tissue slides with tumor cells constituting at least 25% of all cells, after microdissection; one corresponding H&E slide.
Rejection Criteria: Tissue fixed in mercury-containing formalin (eg,Zenker’s) insufficient or undisectable tumor cells in paraffin block; specimens with <25% tumor cells present.
 
Methodology: Tumor DNA is harvested from microdisected unstained FFPE sections. After routine multiplex PCR amplification using software-designated primers, the amplicons are employed in a primer extension step.
Corresponding pathologist’s report must accompany specimen. Formalin-fixed paraffin-embedded (FFPE) unstained slides: 10 unstained sections, each 10-microns thick, corresponding H&E stained 5-micron section required; no mercury based (B-5) fixation.

Container Type

Laboratory Container Default Temperature Minimum Volume
BIOCENTER LAB AP SLIDES Yes 2.0
BIOCENTER LAB STERILE CONTAINER 2.0

Performing Sections

BIOCENTER LAB: MOLECULAR DIAGNOSTICS

Components

Component Name Component ID LOINC Code
IDH1 RESULT 1230002556
IDH2 RESULT 1230002557
INTERPRETATION 1239000531 59462-2 (Clinical biochemist review:Imp:Pt:XXX:Nar:)
MOLDX_FOOTER 12300002393
MOLDX IDH SIGNATURE 12300002509

Synonyms

IDH1 AND IDH2 MUT