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Test Code ACTH Adrenocorticotropic Hormone, Plasma

Reporting Name

Adrenocorticotropic Hormone, P

Useful For

Determining the cause of hypercortisolism and hypocortisolism

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Plasma EDTA


Necessary Information


Separate specimens should be submitted when multiple tests are ordered.



Specimen Required


Patient Preparation: For the 12 hours before specimen collection do not take multivitamins or dietary supplements containing biotin (vitamin B7), which is commonly found in hair, skin, and nail supplements and multivitamins.

Supplies: Aliquot Tube, 5 mL (T465)

Collection Container/Tube: Ice-cooled, lavender top (EDTA)

Submission Container/Tube: Plastic, 5 mL, aliquot tube

Specimen Volume: 1 mL

Collection Instructions:

1. Morning (6 a.m.-10:30 a.m.) specimen is desirable.

2. Collect with a pre-chilled lavender top (EDTA) tube and transport to the laboratory on ice.

3. Centrifuge at refrigerated temperature within 2 hours and immediately separate plasma from cells.

4. Immediately freeze plasma.


Specimen Minimum Volume

0.75 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen (preferred) 28 days
  Refrigerated  3 hours
  Ambient  2 hours

Reference Values

7.2-63 pg/mL (a.m. draws)

No established reference values for p.m. draws

Pediatric reference values are the same as adults, as confirmed by peer reviewed literature.

 

Petersen KE: ACTH in normal children and children with pituitary and adrenal diseases. I. Measurement in plasma by radioimmunoassay-basal values. Acta Paediatr Scand 1981;70:341-345

 

For SI unit Reference Values, see https://www.mayocliniclabs.com/order-tests/si-unit-conversion.html

Day(s) and Time(s) Performed

Monday through Friday; 8 a.m.-10 p.m.

Saturday; 8:30 a.m.-3 p.m.

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

82024

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ACTH Adrenocorticotropic Hormone, P 2141-0

 

Result ID Test Result Name Result LOINC Value
ACTH Adrenocorticotropic Hormone, P 2141-0

Reject Due To

Gross hemolysis Reject
Gross lipemia OK

Method Name

Electrochemiluminescence Immunoassay

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-General Request (T239)

-Oncology Test Request (T729)

Secondary ID

8411