Sign in →

Test Code BNZX Benzodiazepines Confirmation, Chain of Custody, Random, Urine

Specimen Required

Supplies: Chain-of-Custody Kit (T282)

Container/Tube: Chain-of-Custody Kit containing the specimen containers, seals, and documentation required.

Specimen Volume: 20 mL

Collection Instructions: Collect specimen in the container provided, seal, and submit with the associated documentation to satisfy the legal requirements for chain-of-custody testing.

Additional Information: Submitting <20 mL will compromise the ability to perform all necessary testing.


Chain-of-Custody Request is included in the Chain-of-Custody Kit (T282).

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.

Secondary ID


Useful For

Detecting drug use involving benzodiazepines such as alprazolam, chlordiazepoxide, clonazepam, diazepam, midazolam, oxazepam, temazepam, clobazam, flunitrazepam, flurazepam, lorazepam, prazepam, triazolam, and zolpidem, in urine specimens handled through the chain-of-custody process

Additional Tests

Test ID Reporting Name Available Separately Always Performed
COCH Chain of Custody Processing No Yes
ADLTX Adulterants Survey, CoC, U Yes Yes

Testing Algorithm

Testing for adulterants will be performed on all chain of custody urine samples per regulatory requirements.

Method Name

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Benzodiazepines Conf, CoC, U

Specimen Type


Specimen Minimum Volume

5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 7 days
  Frozen  14 days

Reject Due To

Gross hemolysis Reject
Gross icterus Reject

Reference Values



Cutoff concentrations:

Alprazolam by LC-MS/MS: 10 ng/mL

Alpha-Hydroxyalprazolam by LC-MS/MS: 10 ng/mL

Chlordiazepoxide by LC-MS/MS: 10 ng/mL

Clonazepam by LC-MS/MS: 10 ng/mL

7-Aminoclonazepam by LC-MS/MS: 10 ng/mL

Diazepam by LC-MS/MS: 10 ng/mL

Nordiazepam by LC-MS/MS: 10 ng/mL

Midazolam by LC-MS/MS: 10 ng/mL

Alpha-Hydroxy Midazolam by LC-MS/MS: 10 ng/mL

Oxazepam by LC-MS/MS: 10 ng/mL

Temazepam by LC-MS/MS: 10 ng/mL

Clobazam by LC-MS/MS: 10 ng/mL

N-Desmethylclobazam by LC-MS/MS: 10 ng/mL

Flunitrazepam by LC-MS/MS: 10 ng/mL

7-Aminoflunitrazepam by LC-MS/MS: 10 ng/mL

Flurazepam by LC-MS/MS: 10 ng/mL

2-Hydroxy Ethyl Flurazepam by LC-MS/MS: 10 ng/mL

Lorazepam by LC-MS/MS: 10 ng/mL

Prazepam by LC-MS/MS: 10 ng/mL

Triazolam by LC-MS/MS: 10 ng/mL

Alpha-Hydroxy Triazolam by LC-MS/MS: 10 ng/mL

Zolpidem by LC-MS/MS: 10 ng/mL

Zolpidem Phenyl-4-Carboxylic acid by LC-MS/MS: 10 ng/mL

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


G0480 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BNZX Benzodiazepines Conf, CoC, U 90890-5


Result ID Test Result Name Result LOINC Value
608452 Benzodiazepines Immunoassay Screen 14316-4
608280 Alprazolam by LC-MS/MS 59615-5
608281 Alpha-Hydroxyalprazolam by LC-MS/MS 16348-5
608282 Chlordiazepoxide by LC-MS/MS 20522-9
608283 Clonazepam by LC-MS/MS 16229-7
608284 7-aminoclonazepam by LC-MS/MS 51776-3
608285 Diazepam by LC-MS/MS 16227-1
608286 Nordiazepam by LC-MS/MS 16228-9
608287 Midazolam by LC-MS/MS 16233-9
608288 Alpha-Hydroxy Midazolam by LC-MS/MS 59590-0
608289 Oxazepam by LC-MS/MS 16201-6
608290 Temazepam by LC-MS/MS 20559-1
608291 Clobazam by LC-MS/MS 59635-3
608292 N-Desmethylclobazam by LC-MS/MS In Process
608293 Flunitrazepam by LC-MS/MS 20528-6
608294 7-aminoflunitrazepam by LC-MS/MS 51777-1
608295 Flurazepam by LC-MS/MS 16231-3
608296 2-Hydroxy Ethyl Flurazepam by LC-MS/MS In Process
608297 Lorazepam by LC-MS/MS 17088-6
608298 Prazepam by LC-MS/MS 17479-7
608299 Triazolam by LC-MS/MS 16232-1
608300 Alpha-Hydroxy Triazolam by LC-MS/MS 49876-6
608301 Zolpidem by LC-MS/MS 72770-1
608302 Zolpidem Phenyl-4-Carboxylic acid by LC-MS/MS 72768-5
608449 Benzodiazepines Interpretation 16195-0
608450 Chain of Custody 77202-0