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Test Code PNRP Pneumocystis jiroveci, Molecular Detection, PCR, Varies

Reporting Name

Pneumocystis PCR

Useful For

Preferred test for detection of Pneumocystis

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Shipping Instructions

Specimen must arrive within 7 days of collection; specimens older than 7 days will be rejected.

Necessary Information

Specimen source is required.

Specimen Required

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Pneumocystis species DNA is unlikely.


Submit only 1 of the following specimens:



Specimen Type: Body fluid

Sources: Pleural

Container/Tube: Sterile container

Specimen Volume: 1 mL


Specimen Type: Respiratory

Sources: Bronchoalveolar lavage, bronchial washing, tracheal secretions, or sputum

Container/Tube: Sterile container

Specimen Volume: 1 mL


Specimen Type: Tissue

Sources: Respiratory

Container/Tube: Sterile container

Specimen Volume: 5-10 mm

Collection Instructions:

1. Submit fresh tissue.

2. Keep tissue moist with sterile water or sterile saline



Specimen Type: NALC/NaOH-digested respiratory specimens

Sources: Lavage fluid, bronchial washing, respiratory fluid, sputum, or tracheal secretion

Container/Tube: Sterile container

Specimen Volume: 2 mL

Collection Instructions:

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Sunday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
PNRP Pneumocystis PCR 89996-3


Result ID Test Result Name Result LOINC Value
SRC63 Specimen Source 31208-2
81698 Result 89996-3
24188 Special Information 48767-8
24189 Report Status No LOINC Needed

Reject Due To

Other Body fluid other than pleural fluid, blood, bone, nonrespiratory tissue, bone marrow, organ tissues other than lung, paraffin-embedded tissue Specimen in anaerobe vial or viral transport medium Feces Swab Tissue in formalin fluid Urine Specimen >7 days old

Method Name

Real-Time Polymerase Chain Reaction (PCR)


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Secondary ID