Test Code REPU Electrophoresis, Protein, Random, Urine
Advisory Information
Random urine specimens may be sufficient for identifying monoclonal proteins, but 24-hour specimens should be used to quantitate and monitor urinary abnormalities. See MPSU / Monoclonal Protein Study, 24 Hour, Urine.
Shipping Instructions
Refrigerate specimen after collection and send refrigerated.
Necessary Information
Specimen Required
Supplies:
Urine Container, 60 mL (T313)
Aliquot Tube, 5 mL (T465)
Submission Container/Tube: Plastic, 60-mL urine bottle and plastic, 5-mL tube
Specimen Volume: 50 mL
Collection Instructions:
1. Collect random urine specimen.
2. Aliquot at least 25-mL specimen in plastic, 60-mL urine bottle and at least 1-mL of specimen in plastic, 5-mL tube.
3. Label specimens appropriately (60-mL bottle for protein electrophoresis and 5-mL tube for protein, total).
Secondary ID
60068Useful For
Identifying monoclonal gammopathies using random urine specimens
Profile Information
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
RPEU | Protein Electrophoresis, Random, U | No | Yes |
PTCON | Protein, Total, Random, U | No | Yes |
Reflex Tests
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
RIFXU | Immunofixation, Random, U | No | No |
Testing Algorithm
Urine protein electrophoresis alone is not considered an adequate screening for monoclonal gammopathies. If a discrete electrophoresis band is identified, the laboratory will evaluate the urine protein electrophoresis and, if necessary, perform immunofixation at an additional charge.
The following algorithms are available in Special Instructions:
Special Instructions
Method Name
PTCON: Turbidimetry
RPEU: Agarose Gel Electrophoresis
RIFXU: Immunofixation
Reporting Name
Electrophoresis, Protein, Random, USpecimen Type
UrineSpecimen Minimum Volume
25 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Urine | Refrigerated (preferred) | 14 days | |
Frozen | 5 days | ||
Ambient | 72 hours |
Reject Due To
All specimens will be evaluated at Mayo Clinic Laboratories for test suitability. |
Reference Values
PROTEIN, TOTAL
No reference values apply to random urine.
ELECTROPHORESIS, PROTEIN
The following fractions, if present, will be reported as a percent of the total protein:
Albumin
Alpha-1-globulin
Alpha-2-globulin
Beta-globulin
Gamma-globulin
No reference values apply to random urines.
Day(s) and Time(s) Performed
Protein, total: Monday through Sunday; Continuously
Electrophoresis, protein: Monday through Friday; 12 p.m.
Performing Laboratory

Test Classification
This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
84156
84166
86335-Immunofixation (if appropriate)
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
REPU | Electrophoresis, Protein, Random, U | In Process |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
33039 | Albumin | 13992-3 |
33040 | Alpha 1-Globulin | 13990-7 |
33041 | Alpha 2-Globulin | 13993-1 |
33042 | Beta-Globulin | 13994-9 |
33043 | Gamma-Globulin | 13995-6 |
33044 | A/G Ratio | 44293-9 |
33045 | M spike | 42483-8 |
33046 | M spike | 42483-8 |
33047 | Impression | 49299-1 |
PTCON | Protein, Total, Random, U | 2888-6 |