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Test Code REPU Electrophoresis, Protein, Random, Urine

Advisory Information

Random urine specimens may be sufficient for identifying monoclonal proteins, but 24-hour specimens should be used to quantitate and monitor urinary abnormalities. See MPSU / Monoclonal Protein Study, 24 Hour, Urine.

Shipping Instructions

Refrigerate specimen after collection and send refrigerated.

Necessary Information


Specimen Required


Urine Container, 60 mL (T313)

Aliquot Tube, 5 mL (T465)

Submission Container/Tube: Plastic, 60-mL urine bottle and plastic, 5-mL tube

Specimen Volume: 50 mL

Collection Instructions:

1. Collect random urine specimen.

2. Aliquot at least 25-mL specimen in plastic, 60-mL urine bottle and at least 1-mL of specimen in plastic, 5-mL tube.

3. Label specimens appropriately (60-mL bottle for protein electrophoresis and 5-mL tube for protein, total).

Secondary ID


Useful For

Identifying monoclonal gammopathies using random urine specimens

Profile Information

Test ID Reporting Name Available Separately Always Performed
RPEU Protein Electrophoresis, Random, U No Yes
PTCON Protein, Total, Random, U No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RIFXU Immunofixation, Random, U No No

Testing Algorithm

Urine protein electrophoresis alone is not considered an adequate screening for monoclonal gammopathies. If a discrete electrophoresis band is identified, the laboratory will evaluate the urine protein electrophoresis and, if necessary, perform immunofixation at an additional charge.


The following algorithms are available in Special Instructions:

-Laboratory Approach to the Diagnosis of Amyloidosis

-Laboratory Screening Tests for Suspected Multiple Myeloma

Method Name

PTCON: Turbidimetry

RPEU: Agarose Gel Electrophoresis

RIFXU: Immunofixation

Reporting Name

Electrophoresis, Protein, Random, U

Specimen Type


Specimen Minimum Volume

25 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 14 days
  Frozen  5 days
  Ambient  72 hours

Reject Due To

All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Reference Values


No reference values apply to random urine.



The following fractions, if present, will be reported as a percent of the total protein:






No reference values apply to random urines.

Day(s) and Time(s) Performed

Protein, total: Monday through Sunday; Continuously

Electrophoresis, protein: Monday through Friday; 12 p.m.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved or is exempt by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information



86335-Immunofixation (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
REPU Electrophoresis, Protein, Random, U In Process


Result ID Test Result Name Result LOINC Value
33039 Albumin 13992-3
33040 Alpha 1-Globulin 13990-7
33041 Alpha 2-Globulin 13993-1
33042 Beta-Globulin 13994-9
33043 Gamma-Globulin 13995-6
33044 A/G Ratio 44293-9
33045 M spike 42483-8
33046 M spike 42483-8
33047 Impression 49299-1
PTCON Protein, Total, Random, U 2888-6