Test Code TGRP Testosterone, Total and Free, Serum
Reporting Name
Testosterone, Total and Free, SUseful For
Alternative, second-level test for suspected increases or decreases in physiologically active testosterone:
-Assessment of androgen status in cases with suspected or known sex hormone-binding globulin-binding abnormalities
-Assessment of functional circulating testosterone in early pubertal boys and older men
-Assessment of functional circulating testosterone in women with symptoms or signs of hyperandrogenism, but normal total testosterone levels
-Monitoring of testosterone therapy or antiandrogen therapy in older men and in females
Profile Information
Test ID | Reporting Name | Available Separately | Always Performed |
---|---|---|---|
FRTST | Testosterone, Free, S | No | Yes |
TTST | Testosterone, Total, S | Yes | Yes |
Performing Laboratory

Specimen Type
Serum RedOrdering Guidance
This is a second-level test for suspected increases or decreases in physiologically active testosterone. The preferred test for assessment of active testosterone is TTBS / Testosterone, Total and Bioavailable, Serum.
Necessary Information
Patient's age and sex are required.
Specimen Required
Container/Tube: Red top (serum gel/SST are not acceptable)
Specimen Volume: 2.5 mL
Specimen Minimum Volume
1 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum Red | Refrigerated (preferred) | 14 days | |
Frozen | 60 days |
Reference Values
TESTOSTERONE, FREE
Males (adult):
20-<25 years: 5.25-20.7 ng/dL
25-<30 years: 5.05-19.8 ng/dL
30-<35 years: 4.85-19.0 ng/dL
35-<40 years: 4.65-18.1 ng/dL
40-<45 years: 4.46-17.1 ng/dL
45-<50 years: 4.26-16.4 ng/dL
50-<55 years: 4.06-15.6 ng/dL
55-<60 years: 3.87-14.7 ng/dL
60-<65 years: 3.67-13.9 ng/dL
65-<70 years: 3.47-13.0 ng/dL
70-<75 years: 3.28-12.2 ng/dL
75-<80 years: 3.08-11.3 ng/dL
80-<85 years: 2.88-10.5 ng/dL
85-<90 years: 2.69-9.61 ng/dL
90-<95 years: 2.49-8.76 ng/dL
95-100+ years: 2.29-7.91 ng/dL
Males (children):
<1 year: Term infants
1-15 days: 0.20-3.10 ng/dL*
16 days-1 year: Values decrease gradually from newborn (0.20-3.10 ng/dL) to prepubertal levels
*Citation: J Clin Endocrinol Metab 1973;36(6):1132-1142
1-8 years: <0.04-0.11 ng/dL
9 years: <0.04-0.45 ng/dL
10 years: <0.04-1.26 ng/dL
11 years: <0.04-5.52 ng/dL
12 years: <0.04-9.28 ng/dL
13 years: <0.04-12.6 ng/dL
14 years: 0.48-15.3 ng/dL
15 years: 1.62-17.7 ng/dL
16 years: 2.93-19.5 ng/dL
17 years: 4.28-20.9 ng/dL
18 years: 5.40-21.8 ng/dL
19 years: 5.36-21.2 ng/dL
Females (adult):
20-<25 years: 0.06-1.08 ng/dL
25-<30 years: 0.06-1.06 ng/dL
30-<35 years: 0.06-1.03 ng/dL
35-<40 years: 0.06-1.00 ng/dL
40-<45 years: 0.06-0.98 ng/dL
45-<50 years: 0.06-0.95 ng/dL
50-<55 years: 0.06-0.92 ng/dL
55-<60 years: 0.06-0.90 ng/dL
60-<65 years: 0.06-0.87 ng/dL
65-<70 years: 0.06-0.84 ng/dL
70-<75 years: 0.06-0.82 ng/dL
75-<80 years: 0.06-0.79 ng/dL
80-<85 years: 0.06-0.76 ng/dL
85-<90 years: 0.06-0.73 ng/dL
90-<95 years: 0.06-0.71 ng/dL
95-100+ years: 0.06-0.68 ng/dL
Females (children):
<1 year: Term infants
1-15 days: 0.06-0.25 ng/dL*
16 days-1 year: Values decrease gradually from newborn (0.06-0.25 ng/dL) to prepubertal levels
*Citation: J Clin Endocrinol Metab, 36(6):1132-1142, 1973
1-4 years: <0.04 ng/dL
5 years: <0.04-0.07 ng/dL
6 years: <0.04-0.14 ng/dL
7 years: <0.04-0.23 ng/dL
8 years: <0.04-0.34 ng/dL
9 years: <0.04-0.46 ng/dL
10 years: <0.04-0.59 ng/dL
11 years: <0.04-0.72 ng/dL
12 years: <0.04-0.84 ng/dL
13 years: <0.04-0.96 ng/dL
14 years: <0.04-1.06 ng/dL
15-18 years: <0.04-1.09 ng/dL
19 years: 0.06-1.08 ng/dL
TESTOSTERONE, TOTAL
Males
0-5 months: 75-400 ng/dL
6 months-9 years: <7-20 ng/dL
10-11 years: <7-130 ng/dL
12-13 years: <7-800 ng/dL
14 years: <7-1,200 ng/dL
15-16 years: 100-1,200 ng/dL
17-18 years: 300-1,200 ng/dL
≥19 years: 240-950 ng/dL
Tanner Stages*
I (prepubertal): <7-20
II: 8-66
III: 26-800
IV: 85-1,200
V (young adult): 300-950
Females
0-5 months: 20-80 ng/dL
6 months-9 years: <7-20 ng/dL
10-11 years: <7-44 ng/dL
12-16 years: <7-75 ng/dL
17-18 years: 20-75 ng/dL
≥19 years: 8-60 ng/dL
Tanner Stages*
I (prepubertal): <7-20
II: <7-47
III: 17-75
IV: 20-75
V (young adult): 12-60
Day(s) Performed
Monday through Sunday
CPT Code Information
84402
84403
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
TGRP | Testosterone, Total and Free, S | 58952-3 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
3631 | Testosterone, Free, S | 2991-8 |
8533 | Testosterone, Total, S | 2986-8 |
Report Available
3 to 5 daysReject Due To
Gross hemolysis | Reject |
Gross lipemia | Reject |
Gross icterus | Reject |
Method Name
FRTST: Equilibrium Dialysis
TTST: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.